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(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain <t>microvascular</t> <t>endothelial</t> cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.
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(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain <t>microvascular</t> <t>endothelial</t> cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.
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(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain <t>microvascular</t> <t>endothelial</t> cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.
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(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain <t>microvascular</t> <t>endothelial</t> cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.
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(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain <t>microvascular</t> <t>endothelial</t> cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.
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Image Search Results


(a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain microvascular endothelial cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.

Journal: bioRxiv

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion

doi: 10.1101/2025.10.10.681613

Figure Lengend Snippet: (a) Schematics of cytoadhesion assay under static and flow conditions. (b, c) Dot plots showing the number of erythrocytes infected with Pfdgat :LoxPint:HA parasites that adhered to 100 human brain microvascular endothelial cells (HBMECs) under static (b) and flow (c) conditions after 6 days of rapamycin treatment. Data are shown as mean ± SD from n = 3 independent biological replicates (b) and from n = 2 independent biological replicates from 3 independent experiments (c). P values were calculated using one-way ANOVA followed by Tukey–Kramer test. BFA, brefeldin. (d) Dot plots showing the number of SBP1 puncta on infected erythrocytes with Pfdagt :LoxPint:HA on 6 and 8 days of rapamycin treatment. P values were calculated using one-way ANOVA followed by Tukey–Kramer test. Representative immunofluorescence images are shown above the corresponding plots. Samples were stained for SBP1 with rabbit anti-SBP1 antibody (red) and for nuclei with DAPI (blue). Scale bars = 2 μm. was created with Biorender.com. Source data are provided as a Source Data file.

Article Snippet: For the static cytoadherence assay, immortalized human brain microvascular endothelial cells (HBMEC) (P10361-IM; Innoprot, Dario, Spain) were cultivated on 13-mm coverslips (Matsunami Glass, Osaka, Japan) in 24-well plates containing Endothelial Cell Medium (P60104; Innoprot, hereafter referred to as HBMEC medium) supplemented with 5% fetal bovine serum (175012; Nichirei Biosciences, Tokyo, Japan).

Techniques: Infection, Immunofluorescence, Staining